/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-DDX23 Antibody
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Primary Antibodies  

Rabbit anti-DDX23 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Citations (1)
Target:

DDX23

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 1 and 50

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

DDX23 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX23 is a component of the U5 snRNP that may facilitate conformational changes in the splicesome during pre-mRNA splicing.

9416

DDX23

DEAD-box helicase 23

Q9BUQ8

Probable ATP-dependent RNA helicase DDX23

Alternate Names

100 kDa U5 snRNP-specific protein; DEAD (Asp-Glu-Ala-Asp) box polypeptide 23; DEAD box protein 23; probable ATP-dependent RNA helicase DDX23; prp28; PRP28 homolog; PRP28 homolog, yeast; PRP28p homolog; PRPF28; SNRNP100; U5 snRNP 100 kD protein; U5-100K; U5-100KD

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.