NGS (Next-Generation Sequencing) Precision Controls
Next-generation sequencing (NGS) is a technique that allows broad investigation of the genome, with high throughput, scalability and speed. However, one of the biggest challenges with this method is the number of technical errors that can impact the resulting data, from sampling through to analysis.
NGS data interpretation is challenging and prone to workflow errors that are introduced during sample preparation, sequencing, and analysis. These errors can only be partially mitigated by the use of external controls.
External controls come with their own set of challenges including:
- Inability to detect lower target-specific limit of detection (LOD) for each variant and each nucleotide position in each sample, e.g., they don't provide target-specific true LOD
- Inability to detect false positives and false negatives
- Potentially missing clinically actionable calls that are in the noise of the assay
Introducing Accugenomics Internal Spike-In Standards
AccuGenomics SNAQ™-SEQ internal spike-in standards are the ‘Gold Standard’ to achieve accurate clinical sequencing results with full workflow QC, improved sensitivity and per target/sample specificity, elimination of false positives, ability to report copies/ml per variant in liquid biopsy samples, dPCR-like quantification, and significant cost savings.
| External Controls | SNAQ-SEQ Internal Standards | |
| Sample Preparation | Run as a separate sample | Added to every sample |
| Quality Control Check | Run performance of batch reagents only | Sample Specific Performance and Metrics |
| Samples per Flow Cell | Limits # of samples / flow cell | Maximises # of samples / flow cell |
| Cost | Increases cost per run | More cost-efficient runs |
| Background Noise | Difficult to detect false positives and false negatives | Eliminates false positives and false negatives |
| Sensitivity | Limited sensitivity | Per variant LOD per nucleotide position per sample |
SNAQ-SEQ Internal Control Technology
SNAQ-SEQ is a highly customizable spike-in quality control standard for a wide range of NGS applications
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