Rabbit anti-RPA32 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00417 maps to a region between residue 225 and the C-terminus (residue 270) of human Replication Protein A2 (32kD) using the numbering given in SwissProt entry P15927 (GeneID 6118).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RPA32 immobilized on solid support.
The epitope recognized by IHC-00417-T maps to a region between residue 225 and the C-terminus (residue 270) of human Replication Protein A2 (32kD) using the numbering given in SwissProt entry P15927 (GeneID 6118).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
Alternate Names
REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.