Detection of Human PSF/SFPQ by IHC-IF. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PSF/SFPQ (Cat. No. IHC-00304) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus™ 555 (A120-501E) used at a dilution of 1:100.
Detection of Human Vimentin by IHC. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Vimentin (Cat. No. IHC-00314) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).
Detection of Human USP5/IsoT by IHC-IF. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP5/IsoT (Cat. No. IHC-00313) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus™ 555 (A120-501E) used at a dilution of 1:100.
Immunohistochemistry Accessory Kit (Cat. No. IHC-101) provides the reagents and method for sensitive, reliable and economical immunohistochemical staining. This Kit is designed for immunostaining formalin-fixed, paraffin-embedded tissue sections with primary antibodies made in rabbit. It contains all the reagents required for the immunostaining procedure and will stain 250 slides.
Detection of Human Cyclin L2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Cyclin L2 antibody A301-677A used for WB at 0.04 mcg/ml (A) & 0.1 mcg/ml (B) & used for IP at 3 mcg/mg lysate. Cyclin L2 was also immunoprecipitated by Cyclin L2 antibody A301-676A, which recognizes an upstream epitope. For blotting immunoprecipitated Cylcin L2, ReliaBLOT® (Cat. No. WB120) was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of Human EF-1 delta by Western Blot & Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: EF-1 delta antibody A301-684A used for WB at 0.04 mcg/ml (A) and 1.0 mcg/ml (B) and used for IP at 3 mcg/mg lysate. EF-1 delta was also immunoprecipitated by EF-1 delta antibodies A301-683A and A301-685A, which recognize other epitopes. For blotting immunoprecipitated EF-1 delta, ReliaBLOT® (Cat. no. WB210) was used. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).
Detection of Human and Mouse eEF2 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: eEF2 antibody A301-688A used for WB at 0.04 mcg/ml. Detection: Chemiluminescence with exposure time of 1 second.
ReliaBLOT® IP/Western Blot Reagents and Procedures (patent pending) provide an improved method for the detection of immunoprecipitated proteins assayed via Western Blot. It seamlessly integrates into existing IP/WB Protocols with three simple changes. It is optimized for antibodies made in rabbit.
